Fig. 7. PNU anorexigenic action depends on JAK2/STAT3 signalling. 5 mM AG490 (2 µL/mouse), a specific and potent inhibitor of JAK2, was administered by ICV injection. PNU (450 picomol/mouse) was administered by ICV injection 20 minutes later; the hypothalamus was extracted after 40 minutes (saline n = 5, PNU n = 4, AG490+PNU n = 5). (A) AgRP mRNA, (B) NPY mRNA (saline n = 5, PNU n = 4), (C) POMC (saline n = 4, PNU n = 4). STATTIC 30 µM (2 µL/mouse), an inhibitor of STAT3, was administered by ICV injection. PNU (450 picomol/mouse) was administered by ICV injection 20 minutes later; the hypothalamus was extracted after 40 minutes (saline n = 5, PNU n = 4, STATTIC+PNU n= 5). (D) AgRP mRNA, (E) NPY mRNA (saline N = 5, PNU n = 4), (F) POMC (saline n = 4, PNU n = 4). For gene expression analysis, ACTB was used as the endogenous control. One-way ANOVA was used to test differences between groups * p <0.05, ** p <0.01. HYPO=Hypothalamus.